Identifying a new mechanism of sarcopenia by autophagy
Description: We focused on autophagy as a system that can regulate both sarcopenia and metabolic syndrome in skeletal muscle and revealed that non-receptor tyrosine kinase Fyn not only participates in metabolic syndrome but also regulates autophagy regulating sarcopenia through STAT3 regulation, mainly using transgenic mice (Cell metabolism 2010, Cell Rep. 2012).We proceeded with further studies and demonstrating that Fyn-dependent STAT3 phosphorylation by IL6 was observed in mouse C2C12 myotube cells. Autophagy was decreased in those cells by both IL6-dependent and Fyn-dependent mechanisms. Furthermore, in the denervated mouse model, both Fyn and IL6 gene expressions were increased while the autophagy activity was decreased. We next introduced a hind limb suspension mouse model. We found that increased IL6 and STAT3 expression/phosphorylation and decreased autophagy activity. These results strongly suggest that Fyn is involved in the pathogenesis of sarcopenia.
Amount: ¥4,290,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Eijiro Yamada
Institution: Gunma University
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 0
Funder award ID: 18K10987
OpenAlex ID: G894648779