Assessing genome sequence integrity in normal human cells
Description: PROJECT NARRATIVE Human somatic cells are exposed to DNA damaging agents on a daily basis from both endogenous and exogenous sources, resulting in a broad range of DNA lesions. DNA repair of this damage is a complex process, and errors result in DNA mutations. DNA mutations cause cancer and have also been implicated in age-related diseases as well as aging itself. Here we propose to integrate, further optimize and validate two recently developed DNA mutation assays into the first next-generation sequencing-based, comprehensive assay for testing human genome sequence integrity. The assay will then be tested to measuring the mutagenic effects of tobacco smoke in lung, buccal, and blood cells. The assay developed in the proposed project would potentially allow, for the first time, the testing of global genome sequence integrity to assess individual risk of lung cancer in relation to exposure to tobacco smoke, as reflected in non-invasive specimens amenable to epidemiologic studies.
Funding type: cooperative_agreement
Funder scheme: U01
Start date: 2018-08-15
End date: 2023-05-31
Investigator: SIMON D SPIVACK
Institution: ALBERT EINSTEIN COLLEGE OF MEDICINE
Funded outputs count: 30
Funder award ID: U01ES029519
OpenAlex ID: G5700867877