Effect of dietary fat type on plasma lipid profile and leptin concentration in rats fed on high-sucrose diets
Year: 2010
Type: article
Abstract: The aim of the study was to investigate the eff ect of diff erent dietary fat type on plasma leptin concentration and lipid profi le in male Wistar rats fed normo-fat, normo-protein, high-sucrose (5, 19 and 35 % w/w, respectively) diets. The experiment was conducted on 21 adult male rats (260 ± 20g) fed diets with diff erent fat sources: lard (L), grapeseed oil (G) and fl axseed oil (F). Radioimmunoassay was used to measure leptin concentration and enzymaticcolorimetric methods to estimate lipid profi le. Total cholesterol (TC), high density lipoprotein cholesterol (HDL) and triglycerides (TG) plasma concentrations were higher in group L and G than F (ANOVA p≤0,01; p≤0,05 and p≤0,005, respectively), whereas low density lipoprotein cholesterol (LDL) level was higher in group L than G and F (ANOVA p≤0,05). Leptin concentration was signifi cantly higher in group L in comparison to F (ANOVA p≤0,04). Signifi cant positive correlations were found between plasma leptin concentration and fi nal body weight, TC, HDL and TG (r = 0,64, p≤0,006; r=0,72, p≤0,002; r=0,69, p≤0,003; r=0,86, p≤0,00004 respectively). It can be observed that fl axseed oil rich in n-3 polyunsaturated fatty acids (PUFA) profi tably infl uenced not only lipid profi le lowering its parameters but also reduced leptin concentration which can suggest approximate lipidogenic potential of both grapeseed oil (rich in PUFA n-6) and lard (rich in monounsaturated and saturated fatty acids). The results provided evidence that dietary fat type can infl uence cardiovascular disease risk parameters when high-sucrose diet is consumed.
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W1015854962