Work

Destruction of endothelial cells by humoral factors obtained from recipients of xenografts.

Year: 1994

Type: article

Abstract: The moderately difficult model of hamster to rat xenotransplantation has been used by many investigators to study the effects of different immunosuppressive regimens.1-3 Our previous studies have shown that without treatment the graft survival times in this xenotransplant model were 3 days, 6 days, and 7 days for heart, kidney, and liver xenografts, respectively.1-4 We and others have also shown that in this model heart and kidney xenografts were rejected mainly by the humoral factors whereas the liver was rejected by a mixture of cellular and humoral factors.1-4 There are two known mechanisms whereby humoral factors can facilitate the destruction of xenografts, namely C′-dependent Ab-mediated cytotoxicity (CDC) and Ab-dependent cell-mediated cytotoxicity (ADCC).1,5 The purpose of this study was to analyze the induction of humoral factors after heart, kidney, and liver xenotransplantations using hamster aortic endothelial cells (AEC) as targets for CDC and ADCC assays rather than the conventional lymphocyte targets.

Source: PubMed