The use of radiolabelled T1 ribonuclease to monitor the efficiency of Edman degradations (Short Communication)
Year: 1974
Type: article
Abstract: A simple radiolabel tracer method is presented for monitoring the efficiency of the first ten cycles of an Edman degradation. This method consists of adding a very small amount of radiolabelled S-carboxymethylated T(1) ribonuclease to the protein to be sequenced, and counting the radioactivity of a small sample from each of the degradative cycles.
Source: Biochemical Journal
DOI: 10.1042/bj1390779
Authors: David J. McKean, George P. Smith
Institution: University of Wisconsin–Madison
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W103855522