Induced pluripotent stem cells - current progress and their potential for regenerative medicine
Year: 2009
Type: dissertation
Abstract: Both human and mouse somatic cells were reported to be reprogrammed to pluripotent stem\ncells (iPS cells) upon viral transduction with a defined set of transcription factors including\nOCT4, SOX2, KLF4, c-MYC, Nanog and LIN28 in different combinations. Reprogramming\nof somatic cells to pluripotency enables the derivation of patient-specific pluripotent cells that\nhave great potential for regenerative therapies, studying (embryonic) development and the\ndifferentiation process towards different cell lineages. Also, cell lines can be generated to\nmodel genetic diseases and derivatives of these could be used in drug screens and toxicology\ntesting. The use of iPS cells for modeling the development of genetic disorders and their use\nfor transplantation therapies after modifying genetic defects have been successfully shown in\nseveral reports such as in sickle cell anemia, diabetes and Parkinson’s disease. In contrast to\nprevious reprogramming methods, the derivation of iPS cells circumvents the use of oocytes\nor embryonic material. However, the use of oncogenes and integrating viruses for generating\niPS cells should be avoided since this increases the risk of tumor formation. The recent\nfindings that mouse and human iPS cells can be derived with non intregrating vectors and the\nremoval of exogenous reprogramming factors after reprogramming increases the possibility of\nthe future use of iPS cells for clinical applications.
Author: Maaike Welling
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W108752724