Introduction of FRET Application to Biological Single-molecule Experiments
Year: 2013
Type: article
Abstract: Since the fluorescence resonance energy transfer (FRET) measurement is based on the fluorescence measurement technology, the signal can be obtained from single molecules. The single-molecule FRET (smFRET) measurement has already been extensively used in the field of biology. The combination of the single-molecule sensitivity, the nanometer-scale spatial resolution and the realtimeness gives us the exclusive information on the structure and the dynamics of biomolecules, such as coexisting multiple conformational states or temporal evolution of conformational changes. As an introductory review of the smFRET measurement, this article briefly explains the theory, the basic apparatus, the typical data analysis methods and some examples of experiments applied to biomolecules.
Author: Kenji Okamoto
Institution: RIKEN
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W110010569