Work

Telomere G-Overhang Length Measurement Method 1: The DSN Method

Year: 2011

Type: book-chapter

Abstract: Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-092-8_5

Authors: Yong Zhao, Jerry W. Shay, Woodring E. Wright

Institution: The University of Texas Southwestern Medical Center

Language: English

Topic: Telomeres, Telomerase, and Senescence

Subfield: Physiology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Institute on Aging

Award: G2682010215