A Method for Light and Scanning Electron Microscopy of Drought- Induced Damage of Resting Peanut Seed Tissue
Year: 1989
Type: article
Abstract: Tissue damage creates numerous problems for the microscopic examination of resting peanut (Arachis hypogaea L. cv . Florunner) seed physical structure. This paper presents a method to deal with specific problems encountered in light microscopy (LM) and scanning electron microscopy (SEM) of drought - induced damage of peanut tissue . Major findings include: (1) improved SEM imaging through reduction of charging by increasing coating thickness from 25 nm to 30 nm; (2) improved stain affinity for LM tissue preparations by decreasing osmium tetroxide fixation time from 2 hours to 1 hour; and (3) im proved tissue fixation for LM and SE M by application of a modified Karnovsky fixative (glutaraldehyde / formaldehyde combination) which proved more successful than glutaraldehyde alone for tissue preservation.
Authors: Clyde T. Young, William E. Schadel
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
Funder: Agricultural Research Service
OpenAlex ID: W1158811927