Work

Intracellular expression and purification of a recombinant Enzyme involved in bioconversion of Arteannuin b to Artemisinin In Escherichia coli expression system

Year: 2011

Type: article

Abstract: Streptomyces pactum produces an enzyme that converts Arteannuin B to Artemisinin that is an important antimalarial. The gene encoding this novel enzyme from Streptomyces pactum MTCC 3664 has been cloned and expressed in Escherichia coli BL21 (DE3). The enzyme is expressed in the form of inclusion bodies at 37 0 C or in a soluble form at 28 0 C. The recombinant enzyme was purified by Immobilized Metal Ion Affinity Chromatography (IMAC). The purified enzyme produced by constitutive expression was found to be functionally active as it showed the conversion of arteannuin B to artemisinin as detected by TLC and HPLC.

Source: Recent Research in Science and Technology

Authors: Jyotheeswara Reddy Eedula, Parcha Sreenivasa Rao, M. Lakshmi Narasu

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W1201134554

FWCI: 0.269

Cites:

Cited by: 1

Related to: 10