Work

Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue

Year: 1955

Type: article

Abstract: The finding that the acid phosphatase of rat liver is enclosed within a special type of cytoplasmic granules, with sedimentation properties inter- mediate between those of mitochondria and microsomes, has led to the development of a new scheme of fractionation, whereby enzymes attached to these granules can be readily identified (Appelmans, Wattiaux & Duve, 1955).This scheme has been applied in the present work to the study of the following enzymes, previously shown to resemble acid phosphatase in being unequally distributed between mitochondria and microsomes: DPNH- cytochrome c reductase (Hogeboom, 1949; Hoge- boom & Schneider, 1950a;

Source: Biochemical Journal

DOI: 10.1042/bj0600604

Authors: C. de Duve, B. C. Pressman, R. Gianetto, R. Wattiaux, F. Appelmans

Institutions: UCLouvain, KU Leuven

Language: English

Topic: Cancer and biochemical research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W1205458192

FWCI: 28.38

Cites: 39

Cited by: 4,222

Related to: 10