Work

Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

Year: 2011

Type: book-chapter

Abstract: The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-160-4_16

Authors: Charlene Depry, Jin Zhang

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Diabetes and Digestive and Kidney Diseases

Award: Mechanisms of Compartmentalized cAMP Signaling