Work

Xylogenesis and hormones in soybean callus. II. The effect of carbohydrates and various plant growth regulators

Year: 1989

Type: article

Abstract: Xylogenesis was induced in undifferentiated soybean callus by the application of auxin, cytokinins, gibberellic acid and carbohydrates. Auxin (IBA) was a prerequisite for tracheary element formation and its effect could be enhanced significantly by the addition of specific levels of cytokinins, of which kinetin and benzyladenine were more effective than trans-zeatin. A further synergistic effect was achieved when gibberellic acid (10−5 mol dm3) was added to a highly inductive medium containing 0.5 × 10−4 mol dm3 IBA + 10−6 mol dm3 benzyladenine. Of the carbohydrates tested, sucrose at levels of 2 and 3% gave the best results. While autoclaving resulted in the pyrolysis of sucrose, this apparently did not affect xylogenesis adversely. Indications are that at the most inductive hormonal combinations, the sucrose and its hydrolytic products were utilized most rapidly and effectively for tracheary development. Xilogenese in ongedifferensieerde sojaboon-kallus is met sukses met die toevoeging van ouksien, sitokiniene, gibberellien-suur en koolhidrate geïnduseer. Ouksien was ’n voorvereiste vir tracheale element-vorming, maar die effek daarvan is betekenisvol bevorder deur die toevoeging van spesifieke konsentrasies sitokiniene, waarvan kinetien en bensieladenien meer effektief was as trans-zeatin. ’n Verdere sinergistiese effek is waargeneem waar gibberellien-suur (10− 5 mol dm3) gekombineer is met ’n hoogs induktiewe medium wat 0.5 × 10− 4 mol dm3 IBA + 10− 6 mol dm3 bensieladenien bevat het. Sukrose by vlakke van 2 en 3% het die beste resultaat van die getoetste koolhidrate gelewer. Terwyl outoklavering pirolise van sukrose tot gevolg gehad het, het hierdie effek skynbaar nie xilogenese benadeel nie. Daar is aanduidings dat by die mees induktiewe hormonale kombinasies, sukrose en die hidrolitiese produkte daarvan, die vinnigste en mees effektiefste vir tracheale ontwikkeling benut is.

Source: South African Journal of Botany

DOI: 10.1016/s0254-6299(16)31188-7

Authors: Christa Ackermann, J. van Staden

Institution: University of KwaZulu-Natal

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $2,750