Growth and cell-division in extensive (XDR) and extremely drug resistant (XXDR) tuberculosis strains: transmission and atomic force observation.
Year: 2010
Type: article
Abstract: The ultra-structure of Mycobacterium tuberculosis (MTB) was examined by transmission electronic (TEM)) and atomic force microscopy (AFM). The study was performed to describe the morphology of susceptible, multidrug-resistant (MDR), extensively drug-resistant (XDR) and extremely drug-resistant tuberculosis isolates (XXDR-TB) during their exponential growth phase. Four types of cell division were observed and described. While three of them (symmetrical, asymmetrical and branching type) occurred in all isolates studied, the fourth one (adapted type) was seen only in XDR and XXDR-TB bacilli. In the fourth type of cell division, a rod shaped mother cell produced a small round shape bacillus (0.3-0.5 μm). These round cells were different from buds or polar division, but similar to terminal endospores without showing the typing heat resistance. Based on the present observation, we suggest that XDR-and XXDR-TB bacilli accommodate changes helping them to overcome the hostile environment. Viewed under AFM, the other frequently detected shapes in MTB isolates were oval, V, Y and multi-branching filaments. These shape variation confirmed pleomorphic phenomena in MTB populations and the specific features of pan-resistant strains.
Source: PubMed Central
Authors: Parissa Farnia, Reza Masjedi Mohammad, Muayad Aghali Merza, Payam Tabarsi, Gennadii Konstantinovich Zhavnerko, Tengku Azmi Ibrahim, Ho Oi Kuan, Jalladein Ghanavei, Poopak Farnia, Reza Ranjbar, Nikolai Nikolaevich Poleschuyk, Leonid Petrovich Titov, Parviz Owlia, Mehadi Kazampour, Mohammad Setareh, Muaryam Sheikolslami, Giovanni Battista Migliori, Ali Akbar Velayati
Institutions: Universiti Putra Malaysia, Masih Daneshvari Hospital, National Academy of Sciences of Belarus, Baqiyatallah University of Medical Sciences, Republican Research and Practical Center for Epidemiology and Microbiology, Ministry of Health and Medical Education, Istituti Clinici Scientifici Maugeri
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W128647848