Year: 2012
Type: article
Abstract: The absorption of xenobiotics into systemic circulation is impacted by phase II metabolism in the small and large intestine.Enterocytes are absorptive cells in the small intestine that are involved in the metabolism and transport of nutrients and xenobiotics from the lumen to systemic circulation.The inflammatory condition is known to affect endogenous nutrient metabolism and xenobiotic metabolism in the liver.In contrast, minimal attention has been given to the impact of inflammation on the digestion and absorption of dietary compounds.This study examined the influence of an inflammatory environment on the metabolism and distribution of α-mangostin in enterocyte-like Caco-2 cells grown on porous membrane inserts.α-Mangostin belongs to a family of prenylated polyphenols referred to as xanthones that have antioxidant and antiinflammatory activities.Xanthones are relatively abundant in the pericarp of mangosteen fruit.Exposure of highly differentiated Caco-2 cells grown to inflammatory cytokines was associated with decreased expression of uridine diphosphate glucuronsyltransferases 1A (UGT1A) and increased β-glucuronidase activity in Caco-2 cells.Pre-treatment with a pro-inflammatory cocktail altered the metabolism and distribution of α-mangostin in three-compartment culture model.The inflammatory state was associated with an increase in the concentration of free αmangostin in Caco-2 cells and the basolateral compartment compared to control cultures.Exposure to pro-inflammatory cytokines also decreased the amount of phase II metabolites (glucuronidated/sulfated) of α-mangostin in Caco-2 cells and basolateral medium.This reduction in phase II metabolites of α-mangostin is explained, in part, by reduced expression of UGT1A and increased activity of β-glucuronidase in Caco-2 cells.Collectively, these findings suggest that inflammation may alter absorption of α-mangostin.I wish to thank my mother who continues to inspire me to greater heights and is a font of perseverance.I also wish to thank my family, especially my grandparents, as well as my Aunts Gloria and Thelma, who've all instilled within me the love of learning.I am also indebted to the sacrifice my family endured to send me to private school during my formative years.Finally, I want to
Author: Brian Robert Stephens
Institution: The Ohio State University
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W13647371