Work

Target-Decoy Search Strategy for Mass Spectrometry-Based Proteomics

Year: 2009

Type: book-chapter

Abstract: Accurate and precise methods for estimating incorrect peptide and protein identifications are crucial for effective large-scale proteome analyses by tandem mass spectrometry. The target-decoy search strategy has emerged as a simple, effective tool for generating such estimations. This strategy is based on the premise that obvious, necessarily incorrect "decoy" sequences added to the search space will correspond with incorrect search results that might otherwise be deemed to be correct. With this knowledge, it is possible not only to estimate how many incorrect results are in a final data set but also to use decoy hits to guide the design of filtering criteria that sensitively partition a data set into correct and incorrect identifications.

Source: Methods in molecular biology

DOI: 10.1007/978-1-60761-444-9_5

Authors: Joshua E. Elias, Steven P. Gygi

Institution: Harvard University

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences

Awards: New Sample Multiplexing Technologies to Identify Chemical Probes and Illuminate Ubiquitin Biology, G3815886483, INSTITUTIONAL TRAINING GRANT IN GENOMIC SCIENCE, TECHNOLOGY FOR AUTOMATED ANALYSIS OF PROTEOMES, New Technologies for Quantitative Phosphorylation Analysis