Work

Split Gaussia Luciferase for Imaging Ligand–Receptor Binding

Year: 2013

Type: book-chapter

Abstract: Ligand binding to cell surface receptors activates signaling pathways in normal and pathologic conditions, and internalized ligand-receptor complexes may continue to signal from endosomes. Accessibility of cell surface receptors and the central function of ligand-receptor binding in signal transduction make ligand binding a prime target for therapeutic agents. We describe a Gaussia luciferase complementation method for imaging ligand-receptor binding in cell-based assays and living mice. While we illustrate this imaging method for chemokine ligand CXCL12 and its receptors CXCR4 and CXCR7, this imaging strategy can be generalized to a large number of ligand-receptor interactions.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-718-1_5

Authors: Kathryn E. Luker, Gary D. Luker

Institution: University of Michigan

Language: English

Topic: bioluminescence and chemiluminescence research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Cancer Institute