Get started
Work

Aldo-keto reductase family 1 member B8 is secreted via non-classical pathway.

Year: 2014
Type: article
Abstract: Mouse aldo-keto reductase family 1 member B8 (AKR1B8) has the highest similarity to human aldo-keto reductase family 1 member B10 (AKR1B10), a secretory protein through lysosomes-mediated non-classical secretory pathway. To identify whether AKR1B8 is secreted through the same pathway, we carried out this study. Self-developed sandwich ELISA and western blot were used to detect AKR1B8 in cells and culture medium of CT-26 murine colon carcinoma cells. AKR1B8 releases in an independent manner to Brefeldin A, an inhibitor of ER-to-Golgi classical secretion pathway. Several factors, which are involved in the non-classical secretion pathway, such as temperature, ATP and calcium ion, regulated AKR1B8 secretion from mouse colorectal cancer cells CT-26. Lysosomotropic NH4Cl increased AKR1B8 secretion, and AKR1B8 was located in isolated lysosomes. Therefore, AKR1B8 is a new secretory protein through the lysosomes-mediated non-classical pathway.
Authors: Zhenwang Tang, Chenglai Xia, Renbin Huang, Xiaoning Li, Wan-Chun Wang, Wangyuan Guo, Lili Duan, Weihao Luo, Deliang Cao, Di-Xian Luo
Institutions: First Affiliated Hospital of University of South China, Chenzhou First People's Hospital, University of South China, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, SIU Medicine, Second Xiangya Hospital of Central South University
Language: English
Subfield: Cell Biology
Open Access status: green
OpenAlex ID: W1475083171
FWCI: 0.166
Cites: 2
Cited by: 2
Related to: 10