Work

Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function

Year: 2011

Type: article

Abstract: Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function, and dysfunction. We used a Bacterial Artificial Chromosome (BAC) transgenic strategy to express Channelrhodopsin2 (ChR2) under the control of cell-type specific promoter elements. We provide a detailed functional characterization of the newly established VGAT-ChR2-EYFP, ChAT-ChR2-EYFP, TPH2-ChR2-EYFP and Pvalb-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action potential firing of GABAergic, cholinergic, serotonergic, and parvalbumin+ neuron subsets using blue light. This resource of cell type-specific ChR2 mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.

Source: PubMed Central

Authors: Shengli Zhao, Jonathan T. Ting, Hicham Atallah, Li Qiu, Jie Tan, Bernd Gloss, George J Augustine, Karl Deisseroth, Minmin Luo, Ann M. Graybiel, Guoping Feng

Institution: Massachusetts Institute of Technology

Language: English

Topic: Photoreceptor and optogenetics research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

Funders: National Alliance for Research on Schizophrenia and Depression, National Institutes of Health, National Institute of Mental Health

Awards: OptoGenetic Mice for Cell Type-Specific Manipulation of Neuronal Activity, Functional and anatomical characterization of the striosomal system

OpenAlex ID: W1480279010

FWCI: 0.115

Cites: 44

Cited by: 4

Related to: 10