Work

Nucleotide Sequence of a Ribonucleic Acid Transcribed in Vitro from λ Phage Deoxyribonucleic Acid

Year: 1971

Type: article

Abstract: Among the RNA's transcribed from λ b2i21c DNA by a purified preparation of Escherichia coli RNA polymerase is a discrete 6 S species demonstrable by polyacrylamide gel electrophoresis or sucrose density gradient sedimentation. The nucleotide sequence of this RNA is pppApCpGpAp UpGpGpGpUpUpApApUpUpCpGpCpUpCpGpUpUpGpUpGp GpUpApGpUpGpApGpApUpGpApApApApGpApGpGpCpGp GpCpGpCpUp(Up,ApCp)UpApCpCpGpApCpGpUpApUpCpGp UpCpApCpUpUpCpGpApUpUpCpCpGpCpCpUpApGpUpUp GpGpUpCpUpGpGpApApCpUpCpApApCpCpCpApUpCpGp Cp ApGpGp(CpApGpCpGp)CpApGpApGpApGpGpUpCpUpGpCp ApApApApUpGpCpApApUpCpCpCpGpApApApCpApGpUpUp CpGpCpApGpGpUpApApUpApGpUpUpApGpApGpCpCpUpGp CpApUpApApCpGpGpUpUpUpCpGpGpGpApUpUpUpUpUp UpA. This sequence contains three ApUpGp triplets, one of which precedes and is separated by 41 triplets from the hexanucleotide UpApApUpApGp. The sequence permits formulation of extensive regions of base pairing between terminal and internal sequences.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)61977-5

Authors: Paul Lebowitz, Sherman M. Weissman, Charles M. Radding

Institution: Yale University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1480601564

FWCI: 7.996

Cites: 50

Cited by: 180

Related to: 10