Work

Site‐Specific Recombinational Cloning Using Gateway and In‐Fusion Cloning Schemes

Year: 2015

Type: article

Abstract: The comprehensive study of protein structure and function, or proteomics, depends on the obtainability of full-length cDNAs in species-specific expression vectors and subsequent functional analysis of the expressed protein. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence of interest, which differentiates it from classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using the most popular commercial recombinational cloning technologies, Gateway (Life Technologies) and In-Fusion (Clontech).

Source: Current Protocols in Molecular Biology

DOI: 10.1002/0471142727.mb0320s110

Authors: Jaehong Park, Andrea L. Throop, Joshua LaBaer

Institutions: Harvard University, Arizona State University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: Contiunation and Enhancement of the PSI: Biology-Materials Repository