Estimation of folate production by Streptococcus thermophilus using modified microbiological assay.
Year: 2009
Type: article
Abstract: Streptococcus thermophilus, an important dairy starter has been reported to produce appreciable amount of folate during milk fermentation. The microbiological assay, with additional trienzyme treatment, was standardized for the folate estimation and the factors influencing its production by S. thermophilus were evaluated. The microbiological assay was standardized with modifications in respect of order of addition of enzyme (α-amylase, protease and human plasma conjugase) in the tri-enzyme extraction and using Folic Acid Casein media as basal medium. Among the 12 strains screened NCDC 177 was found to produce highest folate (average 34.28 μg/L) followed by NCDC 199 (27.34 μg/L) and NCDC 303 (19.36 μg/L). However, 2 cultures (NCDC 80 and NCDC 312) were found to consume folate from RSM medium. The folate production by NCDC 177 was further enhanced by 62% with incubation at 42° C for 48h and addition of PABA at 100 μM, lactose at 1 % and NaCl at 0.8% concentrations to skim milk.
Language: English
Subfield: Food Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $327
OpenAlex ID: W1486032123