Isolation and characterization of antigen-presenting dendritic cells from the mouse intestinal lamina propria.
Year: 1990
Type: article
Abstract: A method was developed for the isolation of antigen-presenting dendritic cells, and macrophages, from mouse intestinal lamina propria and Peyer's patches. Peyer's patches, and the lamina propria of both the small and large intestine, contained cells with potent stimulatory activity in the allogeneic mixed leucocyte reaction. These cells were separated from macrophages by fibronectin adherence and further enriched by density centrifugation. The isolated stimulatory cells expressed high levels of class II major histocompatibility complex (MHC) antigens, and resembled splenic dendritic cells in both morphology and function. Macrophages were recovered from the lamina propria but not Peyer's patches. These cells also expressed class II MHC antigens, but failed to stimulate the mixed leucocyte reaction and, instead, induced indomethacin-sensitive suppression.
Source: PubMed Central
Authors: P Pavli, C E Woodhams, W F Doe, D A Hume
Institutions: Australian National University, University of Edinburgh
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1490315599