Work

Heterogeneity of human plasma lipoprotein (a). Isolation and characterization of the lipoprotein subspecies and their apoproteins.

Year: 1984

Type: article

Abstract: Lipoprotein (a) (Lp(a] from the plasma of normolipidemic human donors was isolated by rate zonal and isopycnic density gradient ultracentrifugation. The final preparations usually contained varying amounts of isopycnic low-density lipoproteins (LDL), which were totally removed either by heparin-Sepharose column chromatography or by chromatofocusing. The Lp(a) preparations exhibited both inter- and intraindividual density heterogeneity which was accounted for by the differences in their protein and lipid composition. In addition, there was heterogeneity in the size of apoprotein (a) (apo(a] which was found to be linked to apoprotein B (apo-B) through disulfide bonds. Three different apo(a) species were obtained; they had a size either smaller, equal to, or larger than apo-B-100, the protein moiety of LDL. The apo(a) that was smaller than apo-B resided in a low-density Lp(a) particle whose peak was in the 1.019-1.063 g/ml density range. The larger apo(a) was a component of the dense Lp(a) particle and was responsible for the increased density in this Lp(a) species. The third apo(a) which was equivalent in size to apo-B resided in a density range intermediate between the other two Lp(a)s. It is concluded that Lp(a) may differ not only from one individual to another, but also within the same individual who may have more than one Lp(a) species. Part of this heterogeneity may be accounted for by differences in the (a) polypeptide.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)90885-9

Authors: G M Fless, C A Rolih, A M Scanu

Institution: University of Chicago

Language: English

Topic: Lipoproteins and Cardiovascular Health

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430