A Colorimetric Procedure for the Quantitative Determination of Tryptophan Residues in Proteins
Year: 1967
Type: article
Abstract: A simple method for the determination of tryptophan residues in proteins is described. The method involves (a) treatment of the protein with an excess of 2-hydroxy-5-nitrobenzyl bromide, (b) purification of the labeled protein by gel filtration and ethanol washing, (c) measurement of the absorbance at 410 mµ to determine the number of colored groups incorporated, and (d) a determination of the total protein content by conventional methods. The procedure has been shown to be accurate with a wide variety of proteins.
Source: Journal of Biological Chemistry
Authors: T.E. Barman, D.E. Koshland
Institution: University of California, Berkeley
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1492595170