Work

Resolution of factors required for the initiation of transcription by yeast RNA polymerase II.

Year: 1990

Type: article

Abstract: Fractionation of a yeast nuclear extract reveals at least four factors required in addition to RNA polymerase II for accurate initiation of transcription. One of these factors can be replaced by HeLa transcription factor IID or by its yeast counterpart expressed in Escherichia coli. Each of the remaining three factors can be replaced by a fraction from yeast whole cell extract, facilitating further purification of the factors.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)38563-1

Authors: P M Flanagan, R J Kelleher, W J Feaver, N F Lue, J W LaPointe, R D Kornberg

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funder: National Institute of General Medical Sciences