Work

A role for annexin IV in epithelial cell function. Inhibition of calcium-activated chloride conductance.

Year: 1994

Type: article

Abstract: The cellular function of annexin IV was evaluated by correlating tissue expression, cellular localization, and whole-cell electrophysiology. Immunolocalization and biochemical data demonstrate that annexin IV is concentrated along the apical membranes of many epithelia. Introduction of purified exogenous annexin IV into colonic T84 cells through a patch pipette specifically prevented Ca(2+)-dependent Cl- current activation. Affinity-purified antibody against annexin IV applied in the same manner enhanced the activation. Reduction of the endogenous level of annexin IV with a derivatized oligodeoxynucleotide antisense to annexin IV mRNA lowered the threshold for the Ca(2+)-induced current response, mimicking the enhancement of current activation exerted by anti-annexin IV antibody. The inhibitory effect of annexin IV on Ca(2+)-dependent Cl- conductance represents a novel mechanism by which Ca(2+)-binding proteins modulate membrane channel activity.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(17)37687-1

Authors: M.A. Kaetzel, H.C. Chan, W.P. Dubinsky, J.R. Dedman, D.J. Nelson

Institution: University of Cincinnati

Language: English

Topic: S100 Proteins and Annexins

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430