Developing Raman sorting and imaging techniques to characterise and differentiate unculturable microbial single cells
Year: 2012
Type: dissertation
Abstract: Unculturable microorganisms are a major challenge facing researchers in environmental microbiology, and Raman spectroscopy has emerged as a novel technique in this field as it is able to analyse single microbial cells without cultivation.It has been combined with stable isotope probing (SIP) to identify the ecological functions of unculturable microorganisms.However, Raman signal from microorganisms is usually weak and taking single-cell Raman spectra is timing consuming.The interpretation of microorganisms' Raman spectra is also difficult due to their complexity.This thesis aims to improve Raman spectroscopic techniques for environmental microbiology research.Resonance Raman (RR) spectroscopy and surface enhanced Raman scattering (SERS) were employed to enhance single-cell Raman spectra.RR spectroscopy was combined with SIP to
Author: Mengqiu Li
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1503721497