Work

Identification of a 48 S preinitiation complex in reticulocyte lysate.

Year: 1978

Type: article

Abstract: When the polypeptide initiation sequence is interrupted by the peptide antibiotic edeine, the sedimentation coefficient of the native small ribosomal subunit is increased from 43 S to 48 S. Formation of the 48 SN particle is absolutely dependent on the binding of both Met-tRNAf and globin mRNA. These results support the concept that Met-tRNAf binds to the native small ribosomal subunit before mRNA, and that processes involved in mRNA binding to the 43 SN preinitiation complex may be the rate-limiting step of 80 S initiation complex formation.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(17)34810-x

Authors: B. Safer, W. Kemper, R. Jagus

Institutions: National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1505816070

FWCI: 3.005

Cites: 15

Cited by: 47

Related to: 10