Work

Regulation of lipogenesis in isolated hepatocytes by triglyceride-rich lipoproteins.

Year: 1977

Type: article

Abstract: Very low density lipoproteins, chylomicrons, and remnants caused, within an hour, significant inhibition of fatty acid synthesis but not cholesterol synthesis in hepatocytes isolated from meal-fed rats. In contrast, low density lipoproteins, high density lipoproteins, and the serum fraction of density greater than 1.21 failed to significantly inhibit either fatty acid or cholesterol synthesis within 1 h. The Scatchard plots of specific binding showed that rat and human very low density lipoproteins interact with the high affinity sites on the hepatocytes with the apparent dissociation constants of 64 and 106 nM, respectively. These data also indicated that each hepatocyte was capable of binding 6 X 10(5) molecules of very low density lipoproteins.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(17)39885-x

Authors: M R Lakshmanan, R A Muesing, G A Cook, R L Veech

Institutions: George Washington University, St. Elizabeths Hospital, National Institute on Alcohol Abuse and Alcoholism

Language: English

Topic: Cholesterol and Lipid Metabolism

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430