Kidney and adrenal mitochondria contain two forms of NADPH-adrenodoxin reductase-dependent iron-sulfur proteins. Isolation of the two porcine renal ferredoxins.
Year: 1986
Type: article
Abstract: Two NADPH-adrenodoxin reductase-dependent iron-sulfur proteins were detected in both porcine kidney and bovine adrenal mitochondria by using high resolution polyacrylamide electrophoresis.Adrenodoxin (M, = 12,000) constituted the major ferredoxin activity in adrenal mitochondria and a similarly sized protein (M, = 11,500) was isolated as the major renal ferredoxin activity.A second, higher molecular weight ferredoxin was observed in both adrenal (Mr = 13,300) and kidney (Mr= 13,000) mitochondria.The two renal ferredoxins were isolated by the use of ion exchange, gel exclusion, and preparative electrophoretic techniques.An absorption spectrum typical of [2Fe-2S] ferredoxins was obtained for each protein; however, the larger renal molecule had an unusually high 276 nm absorbance.Immunologic studies revealed a significant degree of antigenic commonality between the two renal proteins as well as specific cross-reactivity of adrenodoxin with antiserum raised against the renal proteins.A possible precursor-product relationship between the paired renal and adrenal ferredoxins is discussed.Iron-sulfur proteins are found ubiquitously in nature and typically function as electron-transferring molecules.Adrenodoxin' is one of the most thoroughly characterized iron-sulfur proteins in vertebrate systems.It functions at the inner adrenal mitochondrial membrane to transfer electrons from NADPH-adrenodoxin reductase to cytochromes P-450,, or P-45Ol1,2 (2-4).Adrenodoxin has a molecular mass of approximately 12,000 Da and its [2Fe-%] chromophore imparts a reddish brown color to the protein (5-8).It has a unique optical spectrum with a low 276 nm absorbance and visible maxima at 414 nm and 454 nm (7).The class of * These studies were supported in part by National Institutes of Health Grants AM 32280 and RR 8139.The costs of publication of this article were defrayed in part by the payment of page charges.This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C.Section 1734 solely to indicate this fact.$ T o whom correspondence and requests for reprints should be addressed.The popularized term adrenodoxin will be used synonymously with adrenal ferredoxin.In accordance with the recommendations of the IUPAC-IUB Commission on Biochemical Nomenclature (l), the term renal ferredoxin will denote the iron-sulfur proteins from kidney mitochondria described in this communication. *The abbreviations used are: P-450.,,cholesterol side chain cleavage cytochrome P-450; P-450116, llp-hydroxylase cytochrome P-450; SDS, sodium dodecyl sulfate; PAGE, polyacrylamide gel electrophoresis; ELISA, enzyme-linked immunosorbent assay; Adx-1, -2, bovine adrenodoxin proteins 1 and 2; Rdx-1, -2, pig renal ferredoxin proteins 1 and 2.
Source: Journal of Biological Chemistry
Authors: W J Driscoll, J L Omdahl
Institution: University of New Mexico
Language: English
Field: Energy
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1508167424