Work

A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins

Year: 2008

Type: book-chapter

Abstract: Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate pro tein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpression and copurification of interacting proteins, or in vivo removal of MBP by TVMV protease to improve screening and purification. All target genes and vectors are processed by the same protocols, which we describe here.

Source: Methods in molecular biology

Parent work: High Throughput Protein Expression and Purification: Methods and Protocols

DOI: 10.1007/978-1-59745-196-3_7

Authors: William H. Eschenfeldt, Stols Lucy, Cynthia Sanville Millard, Andrzej Joachimiak, I. Donnelly Mark

Institution: Argonne National Laboratory

Language: English

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Japan Society for the Promotion of Science, National Institute of General Medical Sciences