Work

Construction of a recombinant bacterial plasmid containing pro-alpha 1(I) collagen DNA sequences.

Year: 1980

Type: article

Abstract: A partially purified mRNA preparation enriched for chick collagen messenger RNA activity was used as template for the synthesis of double stranded cDNA. The cDNA was ligated into the HindIII site of the plasmid vector pBR322 and used to transform Escherichia coli x1776. One plasmid with an 800-base pair insert was shown to contain DNA sequences corresponding to Type I pro-alpha 1 collagen.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)85936-7

Authors: T. Yamamoto, M.E. Sobel, S.L. Adams, V.E. Avvedimento, R. DiLauro, I. Pastan, B. de Crombrugghe, A. Showalter, D. Pesciotta, P. Fietzek, B. Olsen

Institutions: Rutgers, The State University of New Jersey, National Institutes of Health, National Cancer Institute, National Cancer Institute, National Cancer Institute, Instituto Nacional del Cáncer

Language: English

Topic: Collagen: Extraction and Characterization

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1511090791

FWCI: 9.645

Cites: 20

Cited by: 59

Related to: 10