Fluorescence immunoassay and passive latex agglutination as alternatives to hemagglutination inhibition for determining rubella immune status
Year: 1983
Type: article
Abstract: Three different assays for detection of rubella antibodies, hemagglutination inhibition (HAI), fluorescence immunoassay (FIA), and passive latex agglutination (PLA) were used to test 297 human serum samples. Overall agreements for immune status were as follows: HAI versus FIA, 95.3% (283 of 297); HAI versus PLA (1:10 dilution), 96.3% (286 of 297); HAI versus PLA (undiluted), 93.9% (279 of 297); PLA (1:10 dilution) versus FIA, 94.9% (282 of 297); and PLA (undiluted) versus FIA, 97.9% (291 of 297). The HAI test is the most time consuming, subjective, and technically difficult to perform. The FIA and PLA tests are very rapid and less labor intensive. In addition, the FIA offers an objective determination of the patient's rubella antibody level.
Source: Journal of Clinical Microbiology
Language: English
Topic: Virology and Viral Diseases
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1511339448