Análisis del patrón global de metilación del DNA en cáncer de mama en mujeres mexicanas
Year: 2012
Type: dissertation
Abstract: Breast cancer is the first leading cause of death from malignant neoplasms in Mexican women. This is due to abnormal growth of cells lining the ducts and lobules of the breast, proliferation is accelerated, disorderly and uncontrolled due to genetic and epigenetic factors. Within the latter, DNA methylation is key in regulating gene expression is altered when it is associated with a variety of malignancies. The imbalance in the DNA methylation pattern was detected mainly in cancer cells, these include the loss of these normally methylated sequences (hypomethylation). Methylation of DNA in cells of breast cancer among Mexican women has been poorly studied in this paper analyzed the methylation pattern by the technique amplification of inter-methylated sites (AIMS / comparative genomic hybridization (PCR-CGH). We used frozen tissue samples of sporadic adenocarcinomas de novo treatment of different stages of breast and surrounding tissue unaffected. To determine intermetilados sites (AIMS) DNA was extracted from tissues by phenolchloroform method, after it was exposed to restriction enzymes Sma I and Xma I and the fragments obtained joined adapters and amplified by PCR . The ensuing comparative genomic hybridization to identify genome-wide methylation changes associated with cancer. DNA probes were labeled control sample and with different fluorophores which were hybridized with metaphase chromosomes, these were visualized using a fluorescence microscope and images were analyzed using the Isis software Axioimager, Karl Zeiss. The search for genes was performed in the database of NCBI Map Viewer. AIMS techniques were standardized, PCR and CGH, and analyzed 3 samples of DNA from tumor tissue and adjacent unaffected. The analysis in software revealed only in the sample of stage II, a hypermethylated region on chromosome 16 near the centromere, to make the comparison in the database for the Map Viewer crosomome it. Several genes as ORCL6, GTP2, DNAJA 2 e ITFG1 gene have been identified in this area, which by function and based in the literature could or associated with breast cancer. The gene described in more information related to cancer is the gene ORCL6, which is involucrate in DNA replication.
Authors: Osorio Martínez, Elia Susana
Language: Spanish
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1511800885