Spectrophotometric Determination of Five Commercial Drugs in Pure Form and Pharmaceutical Formulations by Ion-Pair Complexation with Alizarin Red S
Year: 2015
Type: article
Abstract: Simple, accurate, selective and rapid spectrophotometric methods have been developed for thedetermination of itopride, midodrine, diclofenac, mesalamine and sumatriptan in their pure as well asin pharmaceutical dosage forms. The method was based on ion-pair complex formation between thedrugs and anionic dye, alizarin red S in an acidic medium (pH 2.0-4.0). The complexes formed werequantitatively extracted into chloroform and measured at 284, 287, 279, 285 and 280 nm wavelengthfor itopride, midodrine, diclofenac, mesalamine and sumatriptan respectively. Beer’s law was obeyedin the concentration range of 3.5-35 µg mL-1 for itopride and diclofenac, 2.5-180 µg mL-1 formidodrine, 3.0-80 µg mL-1 for mesalamine and 4.0-200 µg mL-1 for sumatriptan. The stoichiometry ofthe complexes formed between the drugs and the dye was 1:1 as determined by Job’s method ofcontinuous variation. The association constant (KIP) of the ion-pair complexes formed was evaluatedusing Benesi-Hildebrand equation. Limit of detection, limit of quantification and Sandell’s sensitivityof the methods were also estimated. The proposed methods were successfully employed for thedetermination of these drugs in their pharmaceutical dosage forms.
Institution: Gujarat University
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W1512136868