Chemical and Enzymatic Studies of Deoxyribonucleic Acid Covalently Linked to Ficoll
Year: 1972
Type: article
Abstract: Nucleotides and polynucleotides with terminal phosphoryl groups can be covalently linked in a carbodiimide reaction to water-soluble polysaccharides such as Ficoll and dextran. Small, dialyzable nucleotides can be converted to nondialyzable macromolecules of high molecular weight. Hybrid molecules of polynucleotides and polysaccharides have novel properties such as a reduced density in cesium chloride and modified end groups. Nucleotides and polynucleotides attached to Ficoll have been used in enzymatic studies of deoxyribonuclease, polynucleotide kinase, and DNA polymerases.
Source: Journal of Biological Chemistry
Authors: Immo E. Scheffler, Charles C. Richardson
Institution: Harvard University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1512486263