Macromolecular Composition of an Amoeba Plasma Membrane
Year: 1973
Type: article
Abstract: The isolated plasma membrane of Acanthamoeba castellanii consists of approximately 27% lipid, 37% phosphoglycan, and 37% protein. About 60% of the protein migrates as a polypeptide of 15,000 daltons in sodium dodecyl sulfate-acrylamide gel electrophoresis. The phosphoglycan is resolved electrophoretically into two components which migrate ahead of the major polypeptide band. These three components are also detectable upon electrophoretic analysis of whole amoebae and they do not change in concentration when amoeba homogenates are incubated for 6 hours at 25°. These and other controls support the conclusion that the apparently simple macromolecular composition of the amoeba plasma membrane is not an artifact of the isolation procedure. In some preparations actin is associated with the plasma membranes as a nonmembranous component.
Source: Journal of Biological Chemistry
Authors: Edward D. Korn, Paddy L. Wright
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1515931336