Chemical Physiology of the Compound Eye, I.：On the Retinal PAS-Positive Substances (With 1 Text-figure and 4 Plates)
Year: 1962
Type: article
Abstract: This paper is concerned with the distribution of periodic acid-Schiff (PAS) positive substances in the retina of the compound eye and the dynamic state of these substances in relation to the change of photic environment. Material and MethodsThe material used chiefly in this study was females of the commonest house-fly, Musca vicina Macquart which was reared in laboratory on a mixed medium containing sawdust, bean refuse (Okara) and entrails of fishes.Emerged adults were given 5% sucrose solution as a food.For the fixation of PAS positive substances, Carnoy's (abs.alcohol: acetic acid=30: 10 in volume), Pasteels et Leonard's (dioxane saturated with picric acid: formalin: acetic acid = 85: 10: 5), and Gendre's (90% alcohol saturated with picric acid: formalin: acetic acid= 80: 15: 5) solutions were employed; microscopic images of retinal PAS positive substances were confirmed to be considerably different according to these three fixatives.In order to help the permeation of the fixatives, the compound eye was cut in two along the central vertical axis of head under a binocular microscope, and the two half-eyes were immersed into the fixative solutions for a definite time (1.5 hrs. in Carnoy's; 2.5 hrs.
Author: Kenji Suzuki
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1516579842