Year: 2002
Type: article
Abstract: IEX-1, a recently discovered early response gene, regulates cell growth and apoptosis. IEX-1 gene expression is regulated by a variety of factors such as x-irradiation, ultraviolet radiation, steroids, growth factors, and inflammatory stimuli. By systematic examination of the IEX-1 promoter, we show that IEX-1 gene expression is controlled by multiple conserved gene regulatory elements and that IEX-1 is a downstream target of the p53 tumor suppressor and Sp1. In addition, p300, Sox, nuclear factor-kappaB, and AP4 appear to be modulators of IEX-1 gene expression to a lesser degree. We found that there is at least one Sp1 element that functions as an activator and contributes to high basal transcriptional levels of the IEX-1 gene. We demonstrate the presence of a p53 response element that represses IEX-1 promoter activity in HaCaT keratinocytes, indicating that Sp1 and p53 have opposite effects on IEX-1 gene expression. We conclude that IEX-1 expression in cells is regulated by the p53 tumor suppressor and Sp1, thus providing a direct mechanism for control of cell proliferation.
Source: Journal of Biological Chemistry
Authors: Hee-Jeong Im, Mark R. Pittelkow, Rajiv Kumar
Institutions: Mayo Clinic, University of Minnesota Rochester
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
Awards: G1828094709, G3604623962, PATHOGENESIS OF VITAMIN D REFRACTORY DISEASES, Pathological Role of bFGF in Human Adult Articular Cartilage, G4653449631, PATHOGENESIS Of VITAMIN D REFRACTORY DISEASES, G58007902, G7191392185, G7279312517, Role of Vitamin D in Dendritic Cell Immune Functions, THE PHYSIOLOGY AND METABOLISM OF VITAMIN D, THE STRUCTURE OF CALBINDIN-D28K, G9000204832, G9000282168, Mayo Clinic Comprehensive Cancer Center Support Grant, G945531938
OpenAlex ID: W1516909359