Role of Exonuclease and β Protein of Phage λ in Genetic Recombination, V. Recombination of λ DNA in Vitro
Year: 1971
Type: article
Abstract: The sequential action of lambda exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of lambda DNA, whereas the action of ligase alone is insufficient. These results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of lambda exonuclease is arrested when a redundant strand is completely assimilated, and (b) represent a simulation of the steps in genetic recombination that follow the formation of biparental complexes (synapsis). lambda exonuclease is postulated to catalyze a concerted reaction that includes exposure of complementary sequences, formation of heteroduplex regions, and elimination of redundant branches.
Institution: Yale University
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1517540692