Year: 2003
Type: article
Abstract: Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.
Source: Traffic
Authors: Ayano Satoh, Yanzhuang Wang, Jörg Malsam, Matthew B. Beard, Graham Warren
Institution: Yale University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G4438727439, G6242044698
OpenAlex ID: W1519009978