Year: 2015
Type: dissertation
Abstract: Signaling pathways linked to changes in the intracellular cytosolic concentrations of free calcium are involved in a plethora of cellular activities ranging from cell division, differentiation and migration, integration of neural circuits, as well as programmed neuronal cell death. Channels that permit the entrance of calcium into neuronal cells have gathered considerable interest due to their potential of being significant therapeutic targets not least to mention a means by which to answer key neurophysiological questions. This thesis investigates two distinct calcium influx pathways: one mediated by the transient receptor potential channel family which can be either directly activated or indirectly via second messengers and the second pathway involving voltage gated calcium channels. Using a neuronal cell line model (human neuroblastoma IMR-32) as well as the neural progenitor stem cell neurosphere assay with molecular biology techniques, immunocytochemistry, calcium imaging, electrophysiology and time-lapsed imaging the thesis culminates to provide novel insights into the interplay between these channel proteins and the differentiation and migration of neuronal cells. Furthermore it provides new information on the molecular mechanisms involved in the interaction between developing neurons and radial glial cells, whereby the migrational behavior of neuronal cells is influenced by the calcium influx pathways mediated by glutamate receptors in radial glial cells.
Source: Työväentutkimus Vuosikirja
Author: Lauri Louhivuori
Language: English
Topic: Ion Channels and Receptors
Subfield: Sensory Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1519428743