Molecular identification and phenotypic characterization of let-65 (mars-1) in Caenorhabditis elegans.
Year: 2010
Type: dissertation
Abstract: let-65 was first identified in a screen for lethal mutations in C. elegans' unc-22 region.I have demonstrated the molecular identity of let-65 to be F58B3.5 (mars-1), which encodes a methionyl tRNA synthetase (MARS-1).To develop a deeper insight into MARS-1 activity, I experimentally confirmed, from its sub-cellular localization, that it is a cytoplasmic enzyme.I also attempted to determine the sub-cellular localization of every known C. elegans amino-acyl tRNA synthetase using computational methods and, in collaboration with WormBase, renamed the genes appropriately.In addition, I studied let-65 transcription regulation by analyzing its 5' promoter containing region.Haplo-insufficiency phenotypes manifest as a consequence of reduction in copy number of genes that encode proteins involved in translation.I have investigated this in C. elegans.I used two alleles of each of let-65 (mars-1) and let-336 (rps-27) and examined putative haplo-insufficiency phenotypes for both these genes.
Source: Summit (Simon Fraser University)
Author: Maha Alriyami
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W151947736