Work

Purification from human plasma of a heparin-released lipase with activity against triglyceride and phospholipids.

Year: 1975

Type: article

Abstract: A triglyceride lipase different from lipoprotein lipase, but measurable only after intravenous heparin injection, has been isolated from human plasma by sequential use of heparin-Sepharose and concanavalin A-Sepharose affinity chromatography. Using these procedures, phospholipase A1 activity was found to chromatograph identically with the triglyceride lipase. The constancy of the ratio of activities after isoelectric focusing (pI 4.1) and during thermal deactivation indicates that this enzyme has hydrolase activity against both triglycerides and phospholipids. This conclusion was supported further by the homogeneity of the protein as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)40996-4

Authors: C Ehnholm, W Shaw, H Greten, W V Brown

Institutions: University of Helsinki, University of California San Diego

Language: English

Topic: Lipid metabolism and disorders

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430