The intestinal epithelium during damage and regeneration : cell type-specific responses in experimental colitis and after cytostatic drug treatment
Year: 2002
Type: dissertation
Abstract: In the first part of this thesis the role of the colonic epithelium and in particular its associated\nmucus-layer during IBD and in several experimental colitis models is discussed (Chapter 2).\nIn Chapter 3-5 our investigations regarding the colonic epithelium in rat during the different\nphases ofDSS-induced acute colitis are described. Studied are:\nA. clinical symptoms, morphology, proliferation and apoptosis (Chapter 3).\nB. enterocyte-specific gene expression, goblet cell-specific gene expression, and the\nMuc2 - and TFF3 secretion capacity of goblet cells, as a measure of enterocyte\nand goblet cell functioning (Chapters 4 and 5).\nC. goblet cell-specific Muc2 biosynthesis, Muc2 sulfation, and Muc2 secretion\n(Chapter 5).\nThese parameters were studied in conjunction during the onset of disease, active disease and\nthe regenerative phase of DSS-induced colitis, in the proximal and distal colon to obtain\ninsight in the specific colonic functions during damage and regeneration In the second part of this thesis the small intestinal epithelium after treatment with the\ncytostatic drug MTX is investigated. Described are:\nA. clinical symptoms, morphology, proliferation, apoptosis, and enterocyte-, goblet\ncell-, and Paneth cell specific gene expression in the 'normal' small intestinal\nepithelium, i.e. the epithelium distant from the Peyer's Patches (Chapter 6).\nB. the above described parameters in the PP-associated epithelium, and compared\nthese with alterations seen in the small intestinal located more distantly from PP\n(Chapter 7).\nThese parameters were studied on various days after MTX-treatment in the duodenum,\njejunum, ileum, and colon to obtain a complete picture of the epithelial functions during\nMTX-induced damage and regeneration.\nFinally, by comparing the DSS-induced colonic damage with the MTX-induced small\nintestinal damage, the obtained insight in the types of damage and damage control in the colon\nand small intestine are discussed (Chapter 8).
Author: Ingrid B. Renes
Language: English
Topic: Inflammatory Bowel Disease
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: Nestec
OpenAlex ID: W1521204681