Centella asiatica Attenuates Amyloid-β-Induced Oxidative Stress and Mitochondrial Dysfunction
Year: 2015
Type: article
Abstract: BACKGROUND: We previously showed that a water extract of the medicinal plant Centella asiatica (CAW) attenuates amyloid-β (Aβ)-induced cognitive deficits in vivo, and prevents Aβ-induced cytotoxicity in vitro. Yet the neuroprotective mechanism of CAW is unknown. OBJECTIVE: The goal of this study was to identify biochemical pathways altered by CAW using in vitro models of Aβ toxicity. METHODS: The effects of CAW on aberrations in antioxidant response, calcium homeostasis, and mitochondrial function induced by Aβ were evaluated in MC65 and SH-SY5Y neuroblastoma cells. RESULTS: CAW decreased intracellular reactive oxygen species and calcium levels elevated in response to Aβ, and induced the expression of antioxidant response genes in both cell lines. In SH-SY5Y cells, CAW increased basal and maximal oxygen consumption without altering spare capacity, and attenuated Aβ-induced decreases in mitochondrial respiration. CAW also prevented Aβ-induced decreases in ATP and induced the expression of mitochondrial genes and proteins in both cell types. Caffeoylquinic acids from CAW were shown to have a similar effect on antioxidant and mitochondrial gene expression in neuroblastoma cells. Primary rat hippocampal neurons treated with CAW also showed an increase in mitochondrial and antioxidant gene expression. CONCLUSIONS: These data suggest an effect of CAW on mitochondrial biogenesis, which in conjunction with activation of antioxidant response genes and normalizing calcium homeostasis, likely contributes to its neuroprotective action against Aβ toxicity.
Source: Journal of Alzheimer s Disease
DOI: 10.3233/jad-142217
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: U.S. Department of Veterans Affairs, National Institutes of Health, National Institute on Aging, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Complementary and Alternative Medicine, National Institute of Neurological Disorders and Stroke, National Center for Complementary and Integrative Health
Awards: Comprehensive Oregon Neuroscience Network for Excellence in Clinical Trials, G1105865105, Role of oxidative DNA damage in the onset and progression of metabolic syndrome, Mechanisms and active compounds in the cognitive effects of Centella asiatica, CAM Research Training in Neuroscience & Stress, Oregon Alzheimer's Disease Center, G5164910452, G5595008652
OpenAlex ID: W1523394718