Work

The Role of Cobamides in Methionine Synthesis

Year: 1966

Type: article

Abstract: An enzyme has been obtained from extracts of Escherichia coli which catalyzes the synthesis of N5-methyltetrahydrofolate-homocysteine methyltransferase holoenzyme from apoenzyme and 5,6-dimethylbenzimidazolylcobamide deoxyadenosyl in the presence of a reduced pyridine nucleotide. In addition to holoenzyme formation, this system supplies the reducing power for the synthesis of methionine from N5-methyl-H4-folate. It is suggested that this enzyme system functions by reducing the cobamide prosthetic group, and that it may be the natural reducing system required for methionine synthesis in E. coli.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)96660-3

Authors: Nathan Brot, Herbert Weissbach

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: Folate and B Vitamins Research

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1523906215

FWCI: 4.314

Cites: 9

Cited by: 28

Related to: 10