Work

Extended type‐1 chain glycosphingolipid antigens

Year: 1992

Type: article

Abstract: A Lewis-b-active glycosphingolipid containing a repetitive type-1 chain carbohydrate core was isolated from human colonic adenocarcinoma cell line Colo205. This glycosphingolipid was purified by HPLC and preparative high-performance thin-layer chromatography and its structure elucidated by positive-ion fast-atom-bombardment mass spectrometry with collision-induced disassociation, 1H-NMR spectroscopy and methylation analysis. The glycosphingolipid was found to be a trifucosylated derivative of this novel carbohydrate core, having the following structure: [formula; see text].

Source: European Journal of Biochemistry

DOI: 10.1111/j.1432-1033.1992.tb16586.x

Authors: Mark R. STROUD, Steven B. LEVERY, Mary Ellen K. SALYAN, Carl E. ROBERTS, S. HAKOMORI

Institution: University of Washington

Language: English

Topic: Glycosylation and Glycoproteins Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: National Cancer Institute