Behavioral disturbances induced in rats via the intranigral injection of leptin
Year: 2014
Type: article
Abstract: BEHAVIORAL DISTURBANCES INDUCED IN RATS VIA THE INTRANIGRAL INJECTION OF LEPTIN Muratova T. N. Odessa National Medical University, Ukraine Corresponding person: MURATOVA Tatyana Nikolaevna PhD, associate professor, University Clinic of the Odessa National Medical University Tel:+38048-7178916 Fax: +38048-7232215 Mail address: Odessa National Medical University 2, Vallekhovsky Lane, Odessa, 65082, Ukraine e-mail: [email protected] Summary Introduction : Leptin action on brain structures might be helpful for better understanding of mechanism of this hormone neurotropic effects realization. Materials and methods . Experiments have been carried out on Wistar rats (250-320 g) with the help of the EEG-, actometry recording and open field indices registration after microinjection of leptin (5-50 ng) into reticular part of substantia nigra (SNR). Besides, severity of picrtoxon-induced generalized seizures (2 mg/kg, i.p.) have been estimated after intra-SNR leptin administration. Results . It was shown that bilateral intraSNR leptin injection (50 ng) resulted in oligo-akinesia, muscle tonus enhancement, decrease of wakefulness period and increase of deep slow-wave sleep and paradoxal sleep. Unilateral leptin administration (5; 25 and 50 ng) into SNR resulted in the appearance of dose-dependent contralateral circlings, which were blocked by naloxone (1.0 mg/kg). Haloperidol (1.0 mg/kg) caused the potentiation on keeping of uncomfortable position in animals with bilateral intraSNR leptin administration, while naloxone (1.0 mg/kg) markedly reduced this index. Analogous leptin intrastriatal injection failed to induce the same behavioral disturbances. Intranigral leptin administration was followed by decreasing of seizure susceptibility to i.p. picrotoxin administration (2,0 mg/kg). Conclusion: leptin intraSNR induced opiate-depended behavioral disturbances of behavior, and also caused antiseizure action. Key words: leptin, substantia nigra, circlings, naloxone, seizures.
Source: JOURNAL OF HEALTH SCIENCE
Author: Т. Н. Муратова
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1528770029